Record added by Jurg Ott (the original Rockefeller list)

Full Name

Sorting Intolerant From Tolerant


SIFT predicts whether an amino acid substitution affects protein function based on sequence homology and the physical properties of amino acids. SIFT can be applied to naturally occurring nonsynonymous polymorphisms and laboratory-induced missense mutations.


  • Pauling Ng (Genome Institute of Singapore)

  • Steven Henikoff (Fred Hutchinson Cancer Research Institute)



  • Ng, Henikoff (2001), “Predicting deleterious amino acid substitutions”, Genome Research, 11:863-874.

  • Ng, Henikoff (2002), “Accounting for human polymorphisms predicted to affect protein function”, Genome Research, 12:436-446.

  • Ng, Henikoff (2003), “SIFT: Predicting amino acid changes that affect protein function”, Nucleic Acids Research, 31:3812-3814.

  • Kumar, Henikoff, Ng (2009), “Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm”, Nature Protocols, 4:1073-1081.